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- 人、猴
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD64
- 级别：
anti-human CD64 Antibody
- 目录编号：
anti-human CD64 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-human CD64 Antibody
- 抗体英文名：
Alexa Fluor® 700 anti-human CD64 Antibody
- 抗体名：
CD64 Antibody
- 标记物：
anti-human CD64 Antibody
- 宿主：
小鼠
- 适应物种：
人、猴
- 免疫原：
anti-human CD64 Antibody
- 亚型：
anti-human CD64 Antibody
- 形态：
anti-human CD64 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
anti-human CD64 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2100.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥4500.0 |
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is amember of the immunoglobulin superfamily and is expressed on monocytes/macrophages,dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γstimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosisof IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Verified Reactivity ：Human, Cynomolgus, Rhesus
Reported Reactivity ：Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700under optimal conditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
Excitation Laser ：Red Laser (633 nm)
Application Notes ：Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-housetesting suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB).Additional reported applications (for the relevant formats) include: blocking of human IgG3 andmurine IgG2a binding to FcγRI and immunohistochemical staining of a、cetone-fixed frozentissue sections .
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文献和实验1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
光谱，并找到随附的缩窄滤光片，可避免主要的峰值自发荧光。这样做可以大大提升信噪比。 选择量子效率较高的染色剂，或选用浓度更高的造影剂进行实验，可以加强信号，尽管成本更高。现代策略使用近红外（NIR）激发光和 700 纳米以上可激发的标签（如 Cy7 或 Alexa Fluor 750），以避开常见自发荧光体的激发和发射范围（图 2）。 图 2.相对于生物成像中常见的自发荧光分子，Alexa 商业荧光染料的发射峰值。选择在近红外范围内激发和发射的造影剂可以避免自发荧光促成因素造成的不必要的发射。
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
to Streptavidin‐Alexa fluor 532 Reagents and Solutions Commentary Literature Cited Figures Tables
一、如何搭配流式抗体荧光标记 流式抗体荧光标记搭配主要由3个因素决定的：流式细胞仪能检测多少个通道、需要同时检测检测多少个指标、厂家的抗体有多少种荧光标记。如果流式细胞仪的通道越多，那么同一份样本能同时做的表面/胞内标志就越多 A、 如何根据流式细胞仪搭配流式抗体 1） BD流式细胞仪（06版目录第614页） 流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光
