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- Enzo Life Sciences
- 美国
- ENZ-ACC107-0100
- 2025年07月15日
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欣博盛生物
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文献和实验Preparation and Staining of Frozen Tissue Sections
-freezing matrix. Block endogenous peroxidase activity by incubating the slides in 0.3% H2 O2 solution in PBS for 10 minutes. Rinse slides 3x in PBS, 2 minutes each time. Block non-specific binding by incubating with blocking buffer (10
ISH method to visualize miRNA accumulation within individual cells in formalin-fixed paraffin-embedded (FFPE) tissue sections using 5′ and 3′ terminally fluorescein-labeled locked nucleic acid (LNA)-modified probes. We describe a multicolor ISH/IHC
Immunohistochemistry Protocol-Cytokine Antibodies
each, with TBS/0.1% saponin. 4. Block endogenous peroxidase by incubating 30 minutes in TBS/0.3% H2O2/0.1% Saponin, 0.02% NaN3. 5. Wash slides 3X, 3 minutes each, with TBS/saponin. 6. Block non-specific binding sites with 1/100 diluted goat serum in TBS
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