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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
DIGX® Mouse anti-digoxigenin linker (Ready-to-Use)
- 库存:
99
- 供应商:
欣博盛生物
- 保存条件:
+4°C
- 规格:
6 ml
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
for your particular application. Do not use an excess volume of Dynabeads Mouse T-Activator CD3/CD28, as excess Dynabeads per cell may inhibit expansion. Prior to re-stimulation, remove the used Dynabeads by transferring the cells to a suitable tube. Place
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