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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
DIGX® Rabbit anti-digoxigenin linker (Ready-to-Use)
- 库存:
99
- 供应商:
欣博盛生物
- 保存条件:
+4°C
- 规格:
6 ml
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文献和实验and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
实验试剂 Dynal® magnet: see www.invitrogen.com/magnets-selection. Sterile, round-bottomed 2 ml microcentrifuge tubes. Micropipettors and sterile, disposable pipette tips. 65°C water-bath or heating block
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