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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Mouse IgG2bκ Isotype control, Recombinant monoclonal antibody (MOPC-21)
- 应用范围:
Flow Cytometry| WB
- 库存:
99
- 供应商:
欣博盛生物
- 保存条件:
-20°C
- 规格:
200 µg
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文献和实验Isotype Determination of Monoclonal Antibodies by Immunodiffusion
antibodies are allowed to diffuse toward anti-mouse isotype specific antisera in an agarose gel. In a positive reaction, a precipitin line will develop in the agarose between two wells. A monoclonal antibody should show only one heavy chain and one light
Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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