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- 库存：
99
- 英文名：
Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
- 供应商：
北京云肽生物科技有限公司
BioLegend 675508 Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 675508 Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
产品简介：
产品品牌：BioLegend
产品货号：675508
产品名称：BioLegend 675508 Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
产品规格：100 tests
产品说明：
Clone 4B11B69 (See other available formats)
Regulatory Status RUO
Other Names
P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype Mouse IgG2b, κ
描述
ERK1/2是丝氨酸/苏氨酸蛋白激酶的丝裂原活化激酶（MAPKs）的成员。ERK1/2可以通过受体酪氨酸激酶（RTK）、G蛋白偶联受体（GPCR）或蛋白激酶C（PKC）被一系列细胞外刺激激活，如有丝分裂原、生长因子、神经递质、趋化因子和细胞因子。刺激后，ERK1/2被Thr202和Tyr204残基上的上游激酶MEK磷酸化，进而磷酸化参与一系列细胞过程（如细胞增殖、分化、运动和细胞死亡）的许多其他下游分子。
ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
Product Details
Verified Reactivity Human, Mouse
Antibody Type Monoclonal
Host Species Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application ICFC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Antigen Details
Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution Cytoplasm. Nucleus.
Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
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文献和实验相关实验
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
：Catalog NumberProduct NameProduct TypeSizeApplication4408SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F HCA4409SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary
XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂：PBS(磷酸盐缓冲液pH7.4)（实验室常规配置）；非免疫山羊血清；Triton X-100（sigma）；BSA抗体稀释液（实验室常规配置）；A蛋白抗体（Santa cruz）；B蛋白抗体（Abcam）；Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen)；Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
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BioLegend 675508 Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
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