BioLegend 668103 Ultra-LEAF™ P

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BioLegend 668103 Ultra-LEAF™ Purified anti-human CD40
产品简介：
产品品牌：BioLegend
产品货号：668103
产品名称：BioLegend 668103 Ultra-LEAF™ Purified anti-human CD40
产品规格：100 µg

产品说明：
Clone   W17212H (See other available formats)
Regulatory Status   RUO
Other Names
Tumor necrosis factor receptor superfamily member 5, TNFRSF5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Isotype   Rat IgG2b, κ

描述
CD40，也称为TNFRSF5，是一种I型跨膜蛋白，属于TNF受体家族（TNFR）。CD40在B细胞、树突状细胞、单核细胞、巨噬细胞、血小板、基底上皮细胞、内皮细胞和CD34+造血祖细胞亚群上表达。CD40与其配体TNFSF5/CD40L之间的相互作用导致CD40的聚集和TNFR相关因子（TRAFs）的募集，包括TRAF1、2、3、5和6。TRAF1、2、5和6是正调节器，而TRAF3是负调节器。CD40/CD40L相互作用触发两种细胞上的下游信号传导，包括树突细胞上CD40和细胞存活的上调，T细胞上共刺激分子CD80和CD86的上调，以及炎性细胞因子的产生。此外，CD40调节B细胞的发育/成熟、Ig同种型转换，并与其他信号如IL-4结合，促进增殖。据报道，一种转录因子AKNA可以协同调节CD40和CD40L的表达。CD40的失调与多种疾病有关，如3型高IgM免疫缺陷（HIGM3）和霍奇金病。用于癌症治疗的CD40/CD40L靶向性正在临床试验中进行评估，包括抗CD40激动剂抗体和可溶性重组CD40L。已经在人类和小鼠中鉴定出几种同种型。
CD40, also known as TNFRSF5, is a type I transmembrane protein that belongs to the TNF receptor family (TNFR). CD40 is expressed on B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors. The interaction between CD40 and its ligand TNFSF5/CD40L leads to the clustering of CD40 and the recruitment of TNFR associated factors (TRAFs), including TRAF1, 2, 3, 5 and 6. TRAF1, 2, 5 and 6 serve as positive regulators while TRAF3 is a negative regulator. CD40/CD40L interaction triggers downstream signaling on both cells, including upregulation of CD40 and cell survival on dendritic cells, upregulation of co-stimulatory molecules, CD80 and CD86 on T cells, and production of inflammatory cytokines. In addition, CD40 regulates B cell development/maturation, Ig isotype switching and in combination with other signals such as IL-4, promotes proliferation. It has been reported that a transcriptional factor, AKNA, can coordinately regulate the expression of CD40 and CD40L. Dysregulation of CD40 is associated with several diseases, such as hyper-IgM immunodeficiency type 3 (HIGM3) and Hodgkin's disease. Targeting of CD40/CD40L for cancer therapy is being evaluated in clinical trials, including anti-CD40 agonist antibodies and soluble recombinant CD40L. Several isoforms have been identified in humans and mice.

Product Details
Isotype Control  GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl
Verified Reactivity   Human
Antibody Type    Monoclonal
Host Species   Rat
Immunogen    Human CD40 Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application   Block - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by blocking the binding between hCD40 and hCD40L. The ND50 range: 0.2 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure   Disulfide bond-linked homodimer
Distribution
B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34 hematopoietic progenitors, primary carcinomas.+

Function
CD40 regulates B cell development/maturation, Ig isotype switching proliferation. CD40 is also involved in T cell priming and inflammatory cytokine production.
Interaction    TRAF1, 2, 3, 5, 6
Ligand/Receptor     TNFSF5/CD40L
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Platelets

Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors

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