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- BioLegend
- 2025年12月25日
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99
- 英文名：
BioLegend 663104 Purified anti-E. coli RNA Polymerase α
- 供应商：
北京云肽生物科技有限公司
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 663104 Purified anti-E. coli RNA Polymerase α
产品简介：
产品品牌：BioLegend
产品货号：663104
产品名称：BioLegend 663104 Purified anti-E. coli RNA Polymerase α
产品规格：100 µg
产品说明：
Clone 4RA2 (See other available formats)
Regulatory Status RUO
Other Names
RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez
Isotype Mouse IgG1
描述
DNA依赖性RNA聚合酶利用四种核糖核苷三磷酸作为底物催化DNA转录为RNA。该亚基在亚基组装中起着重要作用，因为其二聚化是亚基顺序组装形成全酶的第一步。
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.
Product Details
Verified Reactivity
E. coli, Widely among Gram-negative bacteria, some Gram-positive bacteria
Antibody Type Monoclonal
Host Species Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration 0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application WB - Quality tested
Recommended Usage
Each lot of antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 ug/mL (1:500-1:5000). It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
Structure
α is a 36 kD subunit of the E. coli RNA polymerase.
Biology Area Cell Biology, Transcription Factors
Molecular Family Nuclear Markers
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文献和实验Sauer:RNA Purification from E. coli
My Experience Purifying RNA from E. coli Regarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria. With Qiagen (anion exchange) or other silica-binding technologies, the smaller RNAs
Sauer:Lysing E. coli with Lysozymes
Getting The Most Out Of Your Bugs Native lysis is a staple protocol in practically every biochemistry lab, yet there is significant variability in the procols I have read. The intention is to liberate the guts of E. coli
is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
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