BioLegend 655701 Purified anti

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BioLegend 655701 Purified anti-IRF3
产品简介：
产品品牌：BioLegend
产品货号：655701
产品名称：BioLegend 655701 Purified anti-IRF3
产品规格：25 µg

产品说明：
Clone  12A4A35 (See other available formats)
Regulatory Status  RUO
Other Names   Interferon regulatory factor-3 (IRF-3)
Isotype   Mouse IgG2b, κ

描述
IRF-3是干扰素调节转录因子（IRF）家族的成员。它介导干扰素刺激反应元件（ISRE）启动子的激活，并作为抗病毒活性的分子开关发挥作用。在病毒感染过程中产生的DsRNA导致C末端丝氨酸/苏氨酸簇上的IRF-3磷酸化。这诱导了构象变化，导致其二聚化、核定位并与CREB蛋白结合到dsRNA激活因子1，dsRNA激活因素1是一种在ISRE控制下激活基因转录的复合物。磷酸化的IRF-3受到泛素介导的降解的负调控。研究表明，它在刺激TLR介导的IL-27 p28基因表达中起着关键作用。
IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.

Product Details
Isotype Control   Purified Mouse IgG2b, κ Isotype Ctrl
Verified Reactivity   Human, Mouse
Antibody Type   Monoclonal
Host Species   Mouse
Immunogen
Partial IRF-3 recombinant protein (200-427 a.a.)

Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation   Affinity purified
Concentration   0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application   WB - Quality tested
ChIP - Verified

Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure
Human IRF-3 has 427 amino acids with predicted Mw 47.2kD and observed Mw 52kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9kD and observed Mw 45kD.

Distribution
Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.

Function
IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.

Interaction
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.

Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family    Nuclear Markers

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