BioLegend 644816 Brilliant Vio

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BioLegend 644816 Brilliant Violet 421™ anti-T-bet
产品简介：
产品品牌：BioLegend
产品货号：644816
产品名称：BioLegend 644816 Brilliant Violet 421™ anti-T-bet
产品规格：500 µL

产品说明：
Clone   4B10 (See other available formats)
Regulatory Status  RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype  Mouse IgG1, κ

描述
T-bet，也称为T-box转录因子T-bet被认为是Th1淋巴发育的“主调节因子”，控制细胞因子IFN-γ的产生。T-bet在造血细胞中广泛表达，包括干细胞、NK细胞、B细胞和T细胞。T-bet对控制微生物病原体至关重要，敲除动物表现出哮喘特有的多种生理和炎症特征。T-bet表达在IL-12刺激后最佳地观察到，并且可以通过添加Th2细胞因子IL-4或中和IL-12来抑制。
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Isotype Control
Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
Verified Reactivity   Human, Mouse
Antibody Type   Monoclonal
Host Species   Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICFC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Antigen Details
Structure
T-box transcription factor, approximately 58 kD.
Distribution
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.

Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family   Nuclear Markers

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