BioLegend 622205 PE anti-mouse

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BioLegend 622205 PE anti-mouse TIGIT (Vstm3)
产品简介：
产品品牌：BioLegend
产品货号：622205
产品名称：BioLegend 622205 PE anti-mouse TIGIT (Vstm3)
产品规格：25 µg

产品说明：
Clone   A17200C (See other available formats)
Regulatory Status   RUO
Other Names
T-Cell Immunoreceptor with Ig and ITIM domains, T cell Ig, Immunoglobulin Domain-Containing Protein 9, V-Set, Transmembrane Domain-Containing Protein 3, WUCAM
Isotype   Rat IgG2a, κ

描述
TIGIT（具有Ig和ITIM结构域前体的T细胞免疫受体）包含免疫球蛋白可变结构域、跨膜结构域和基于免疫受体酪氨酸的抑制基序。TIGIT是一种在自然杀伤细胞（NK）表面表达的抑制性受体。TIGIT识别连接蛋白和连接蛋白样粘附分子，因此在恶性转化的先天免疫反应中发挥关键作用。TIGIT与CD226结合形成与共刺激CD28/CTLA-4通路具有显著相似性的通路。CD226和TIGIT结合同一组配体（CD155和CD112），CD226是T细胞反应的阳性调节因子，而TIGIT抑制它们。人CD4+T细胞中TIGIT表达的敲除导致T-bet和IFN-γmRNA和蛋白质表达的增加。这些作用通过阻断CD226信号传导而被克服，这表明TIGIT通过与CD226竞争同一配体CD155而发挥免疫抑制作用。CD155与人树突状细胞上的TIGIT的结合增强了白细胞介素10的产生，并减少了白细胞中介素12p40的产生。TIGIT在实验性自身免疫性脑脊髓炎（EAE）的小鼠模型中显示出对细胞信号通路的抑制作用。小鼠TIGIT表达的缺失导致过度增殖的T细胞反应和对EAE的易感性增加。
TIGIT (T cell immunoreceptor with Ig and ITIM domains precursor) contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif. TIGIT is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. TIGIT in conjunction with CD226 forms a pathway that has prominent similarities to the costimulatory CD28/CTLA-4 pathway. CD226 and TIGIT bind the same set of ligands (CD155 and CD112) and CD226 is a positive regulator of T cell responses, while TIGIT inhibits them. Knockdown of TIGIT expression in human CD4+ T cells results in an increase of both T-bet and IFN-γ mRNA and protein expression. These effects are overcome by blocking CD226 signaling indicating that TIGIT exerts immunosuppressive effects by competing with CD226 for the same ligand, CD155. The binding of CD155 to TIGIT on human dendritic cells enhances the production of interleukin 10 and diminishes the production of interleukin 12p40. TIGIT shows an inhibitory effect on cell-signaling pathway in murine models of experimental autoimmune encephalomyelitis (EAE). Loss of TIGIT expression in mice results in hyperproliferative T cell responses and increased susceptibility to EAE.

Product Details
Isotype Control   PE Rat IgG2a, κ Isotype Ctrl
Verified Reactivity   Mouse
Antibody Type   Monoclonal
Host Species   Rat
Immunogen   Mouse TIGIT Recombinant Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration   0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure   Homodimer
Distribution
Human follicular B helper T cells (TFH), regulatory, memory and activated T cells, NK cells.

Function
Immunosuppressive effects by binding CD155 and modulating cytokine production by DC. Inhibits NK cell cytotoxicity. Inhibits T cell functions by competing with CD226. Tregs TIGIT cells suppress proinflammatory Th1 and Th17 cells, but not Th2 cell responses. +

Interaction   Dendritic cells, monocytes
Ligand/Receptor
Poliovirus receptor (PVR/CD155) and nectin-2 (PRR-2/CD112)
Cell Type   NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors

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