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文献和实验Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells
molecules CD40, CD80, and CD86 (5 ,6 ), and production of cytokines such as interleukin-12 (IL-12) (7 -9 ), IL-6 (10 ), and interferon α (IFN-α) (10 ,11 ). Moreover, DC can process antigens in various forms by different pathways and induce high levels
Collection, Separation, and Activation of Human T Lymphocytes
and is constitutively expressed on the surface of both CD4+ and CD8+ T lymphocytes (5 ,6 ). CD28 and its homolog CTLA-4 bind the natural ligands B7.1 and B7.2 (also known as CD80 and CD86, respectively), which are present on antigen presenting cells (APCs
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
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