背景介绍: Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
免疫原: Recombinant full length protein of active Rab11
经过测试的应用: IP, IHC, IF
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
浓度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亚型: IgG2b
纯度: Purified from ascites
防腐剂: No
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: Anti-Rab11-GTP monoclonal antibody recognizes active Rab11 from vertebrates.
储存条件: Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:
Rab11 Activation Assay. Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. TD-26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. ).