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- 详细信息
- 技术资料
- 供应商:
Bioyears
- 库存:
88
- 靶点:
Rac1
- 级别:
科研级
- 目录编号:
TD-26903
- 克隆性:
单克隆
- 抗原来源:
蛋白表达
- 保质期:
24个月
- 抗体英文名:
Anti active Rac Mouse Monoclonal Antibody
- 抗体名:
Rac1-GTP
- 标记物:
FITC
- 宿主:
Mouse
- 适应物种:
脊椎动物
- 免疫原:
Recombinant full length protein of active Rac1
- 亚型:
IgM
- 形态:
液体
- 应用范围:
IP, IHC IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- 保存条件:
-20℃
- 浓度:
1mg/ml
- 规格:
30μl/5*30μl
| 规格: | 30μl | 产品价格: | ¥4800.0 |
|---|---|---|---|
| 规格: | 5*30μl | 产品价格: | ¥20000.0 |
Rac1-GTP
| Cat. #: TD-26903 |
| Size: 30 µL |
| Gene Symbol: RAC1 |
| Description: Anti-Rac1-GTP Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| Immunogen: Recombinant full length protein of active Rac1 |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgM |
| Purity: Purified from ascites |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: ![]() |
Immunofluorescence: ![]() Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [ TD-26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ºC methanol or 4% parafo/rmaldehyde (fixation time 1 hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1:1000. |
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Rac1-GTP
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