BioLegend 571309 Recombinant Human IL-22 (ELISA Std.) 产品简介: 产品品牌:BioLegend 产品货号:571309 产品名称:BioLegend 571309 Recombinant Human IL-22 (ELISA Std.) 产品规格:4 pack
产品说明: Regulatory Status RUO Other Names TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
描述 IL-22是一种在结构上与IL-10相关的细胞因子。IL-22最初被鉴定为IL-9在T细胞和肥大细胞中诱导的小鼠基因,最初被命名为ILTIF(IL-10相关T细胞衍生的诱导因子)。IL-22属于一个与IL-10同源性有限的细胞因子家族,即IL-10、IL-19、IL-20、IL-24、IL-26,以及IFN-λs、IL-28A、IL-28B和IL-29。人IL-22与鼠IL-22具有79%的氨基酸同一性,与人IL-10具有25%的同一性。IL-22的生物活性是通过与由IL-22R1和IL-10R2受体链组成的细胞表面复合物结合而启动的。活性通过与可溶性结合蛋白IL-22BP的相互作用进一步调节,IL-22BP与IL-22R1的细胞外区域(sIL-22R1)具有序列相似性。IL-22R复合物的两条链都属于II类CRF。已经发现了两种类型的IL-22结合受体,一种是膜结合受体,另一种是可溶性受体,两者都由不同的基因编码。IL-22由Th17细胞和新鉴定的Th22细胞产生。除了在自身免疫性疾病中观察到的失调炎症外,它在粘膜免疫中发挥着关键作用。 IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
Product Details Source Human IL-22, amino acids Ala34-Ile179 (Accession# NM_020525) was expressed in E. coli.
Molecular Mass The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.88 kD. The DTT-reduced protein migrates at approximately 15 kD and the non-reduced protein migrates at approximately 14 kD by SDS-PAGE.
Purity Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, and protease inhibitors.
Endotoxin Level Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles. Application ELISA - Quality tested
Recommended Usage Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 8000 pg/ml to 125 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details Structure Cytokine Distribution Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
Function Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosisInteractionInteracts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor IL-22R1 and IL-10R2 receptor chainsBioactivityIL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cellsBiology AreaStem Cells, Immunology, Cell Biology Molecular Family Cytokines/Chemokines