BioLegend 559404 Recombinant Human RANK (TNFRSF11A) (carrier-free) 产品简介: 产品品牌:BioLegend 产品货号:559404 产品名称:BioLegend 559404 Recombinant Human RANK (TNFRSF11A) (carrier-free) 产品规格:25 µg
产品说明: Regulatory Status RUO Other Names Receptor activator of NF-KB, Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), Osteoclast differentiation factor receptor (ODFR), TNF-related activation-induced cytokine receptor (TRANCER), Osteosarcoma tumor supresor (OTS), CD265
描述 RANK最初是从树突状细胞中克隆出来的。它是一种I型膜66kD蛋白,在单核细胞、巨噬细胞、树突状细胞、破骨细胞前体和其他细胞中表达。RANK及其配体(RANKL)在骨稳态、器官发生、免疫耐受和癌症中起着关键作用。RANK和RANKL是TNF家族的成员,分别命名为TNFRSF11A和TNFSF11。RANK和其他TNF受体成员一样,缺乏内在的酶活性。其信号转导是通过募集各种衔接蛋白来进行的,包括TNFR相关因子(TRAFs),从而激活JNK、ERK、p38、NFATc1、AKT和NF-κB信号通路。此外,IFN-γ、IFN-β和ITAM激活的共刺激信号与RANK信号相互作用,以相反的方式调节破骨细胞生成。当IFN-γ和IFN-β抑制时,ITAM刺激这一过程。RANK及其配体与骨疾病有关,如绝经后骨质疏松症和炎症条件下的骨丢失。RANKL在活化的T细胞上表达可诱导类风湿性关节炎的骨丢失和关节破坏。此外,RANK在髓质胸腺上皮细胞(mTEC)发育过程中提供信号。mTEC有助于免疫抑制性Foxp3阳性调节性T细胞的选择和存活。 RANK was initially cloned from dendritic cells. It is a type I membrane 66 kD protein that is expressed in monocytes, macrophages, dendritic cells, osteoclast precursors, and among other cells. RANK and its ligand (RANKL) play a key role in bone homeostasis, organogenesis, immune tolerance, and cancer. RANK and RANKL are members of the TNF family and are respectively designated as TNFRSF11A and TNFSF11. RANK, as well as other TNF receptor members, lacks intrinsic enzymatic activity. Its signal transduction is carried out by the recruitment of various adaptor proteins, including TNFR-associated factors (TRAFs), leading to the activation of JNK, ERK, p38, NFATc1, AKT, and NF-κB signaling pathways. In addition, IFN-γ, IFN-β, and ITAM-activated costimulatory signals crosstalk with RANK signaling to regulate osteoclastogenesis in the opposite manner. While IFN-γ and IFN-β inhibit, ITAM stimulates this process. RANK and its ligand are associated to bone diseases such as postmenopausal osteoporosis and bone loss in inflammatory conditions. RANKL expressed on activated T-cells induces bone loss and joint destruction in rheumatoid arthritis. Also, RANK provides signals during medullary thymic epithelial cells (mTEC) development. mTECs contribute to the selection and survival of immunosuppressive Foxp3-positive regulatory T cells.
Product Details Source Human RANK, amino acids (Gln29-Gly213) (Accession# AF018253), was expressed in 293E cells with a C-terminal containing IgG-Fc-His tag.
Molecular Mass The 191 amino acid recombinant protein has a predicted molecular mass of approximately 47.8 kD. The protein migrates at approximately 60 kD in DTT-reduced conditions and at approximately 120 kD in non-reduced conditions by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity >95%, as determined by Coomassie stained SDS-PAGE.
Formulation 0.22 µm filtered protein solution is in PBS, pH 7.2. Endotoxin LevelLess than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity Human RANK inhibits the osteoclast differentiation induced by RANKL (Cat. No. 591102) at 20 ng/mL in a dose-dependentmanner in the presence of anti-His antibody (Cat. No. 652507). The ED50 is 6 - 30 ng/mL. Application Bioassay
Application Notes BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details Distribution Monocyte/macrophage lineage, dendritic cells, osteoclast precursors, mature osteoclasts, neurons and astrocytes of the preoptic area, and medial septal nucleus in the hypothalamus.
Function RANK is a key regulator of bone remodeling, lymph node organogenesis, formation of lactating mammary gland, and body temperature. RANK is induced by M-CSF on osteoclast precursor cells.
Interaction Bone marrow stromal cells, osteoblasts, and activated T cells. Ligand/Receptor RANKL/TNFSF11.
Bioactivity Soluble RANK inhibits osteoclast differentiation on RAW246.7 murine macrophages. Cell Type Embryonic Stem Cells Biology Area Cell Biology, Immunology, Stem Cells Molecular Family CD Molecules, Cytokines/Chemokines, Growth Factors