由于磁珠的特性,MojoSort™试剂通常需要稀释才能在色谱柱分离器上获得最佳使用效果。在可用的情况下,根据内部测试,每种试剂盒组分的推荐稀释因子在网页的“应用说明”部分提供。 Non CD3+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD3+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Preparation The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C. Application Cell Separation (MojoSort™) - Quality tested
Recommended Usage 10 µl of antibody cocktail for 1x107 cells in 100 µl of buffer. 10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer. For this kit, incubation with antibody cocktail and Streptavidin Nanoparticles can be done for 5 to 15 minutes, without loss in purity and yield.
Antigen Details Biology Area Immunology Molecular Family CD Molecules, TCRs