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- 英文名：
BioLegend 480131 MojoSort™ Human CD3 T Cell Isolation Kit
- 供应商：
北京云肽生物科技有限公司
BioLegend 480131 MojoSort™ Human CD3 T Cell Isolation Kit
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 480131 MojoSort™ Human CD3 T Cell Isolation Kit
产品简介：
产品品牌：BioLegend
产品货号：480131
产品名称：BioLegend 480131 MojoSort™ Human CD3 T Cell Isolation Kit
产品规格：200 tests
产品说明：
描述
通过将样品与生物素抗体混合物孵育，然后与磁性链霉亲和素纳米珠孵育来耗尽非 CD3+ T 细胞。磁性标记的部分通过使用磁选机保留。通过在干净的管中倾析液体来收集未接触的CD3 + T细胞。这些是您感兴趣的单元格;请勿丢弃液体。一些下游应用包括功能测定、基因表达、表型表征等。
MojoSort™试剂还与其他供应商提供的基于柱的细胞分离系统兼容。在“相关方案”部分为每个试剂盒提供了使用内部测试色谱柱进行细胞分离的优化方案，并在产品网页（如果有）上提供了代表性数据。使用列分隔符生成的数据在图例中指示。
由于磁珠的特性，MojoSort™试剂通常需要稀释才能在色谱柱分离器上获得最佳使用效果。在可用的情况下，根据内部测试，每种试剂盒组分的推荐稀释因子在网页的“应用说明”部分提供。
Non CD3+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD3+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Product Details
Verified Reactivity Human
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application Cell Separation (MojoSort™) - Quality tested
Recommended Usage
10 µl of antibody cocktail for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.
For this kit, incubation with antibody cocktail and Streptavidin Nanoparticles can be done for 5 to 15 minutes, without loss in purity and yield.
Antigen Details
Biology Area Immunology
Molecular Family CD Molecules, TCRs
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 344104 PE anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344106 APC anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344109 PE/Cyanine7 anti-human CD141 (Thrombomodulin) M80 25 tests
BioLegend 344110 PE/Cyanine7 anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344116 Brilliant Violet 785™ anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344117 Brilliant Violet 605™ anti-human CD141 (Thrombomodulin) M80 25 tests
BioLegend 344118 Brilliant Violet 605™ anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344124 Alexa Fluor® 647 anti-human CD141 (Thrombomodulin) M80 100 tests
BioLegend 344302 Purified anti-human CD88 (C5aR) S5/1 100 ug
BioLegend 344304 PE anti-human CD88 (C5aR) S5/1 100 tests
BioLegend 344306 FITC anti-human CD88 (C5aR) S5/1 100 tests
BioLegend 344505 PE anti-human CD272 (BTLA) MIH26 25 tests
BioLegend 344514 PerCP/Cyanine5.5 anti-human CD272 (BTLA) MIH26 100 tests
BioLegend 344515 PE/Cyanine7 anti-human CD272 (BTLA) MIH26 25 tests
BioLegend 344518 APC/Cyanine7 anti-human CD272 (BTLA) MIH26 100 tests
BioLegend 344520 Alexa Fluor® 647 anti-human CD272 (BTLA) MIH26 100 tests
BioLegend 344602 Purified anti-human CD4 SK3 100 ug
BioLegend 344606 PE anti-human CD4 SK3 100 tests
BioLegend 344607 PerCP/Cyanine5.5 anti-human CD4 SK3 25 tests
BioLegend 344608 PerCP/Cyanine5.5 anti-human CD4 SK3 100 tests
BioLegend 344610 Biotin anti-human CD4 SK3 100 ug
BioLegend 344611 PE/Cyanine7 anti-human CD4 SK3 25 tests
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文献和实验相关实验
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息： MojoSort™ Human CD4 T Cell Isolation Kit（BioLegend, Cat#480009），阴性分选试剂盒2.检测仪器型号：BD LSRⅡ3.样本：正常人外周血4.分选前后检测相关产品：PE-CD4（Biolegend, Cat# 317410）5.实验过程（1）采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。（达优，Cat#DKW-KLSH-0100
上 Strep-tag 的 Fab 片段（Fab-Streps）特异性的结合到琼脂糖基质上。随后，加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上，其他非目的细胞则有效的被洗去。最后，加入生物素使目的细胞从 Fab-Streps 上解离。至此，就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品： CD3 Fab-TACS® Gravity Kit, human（IBA Lifesciences, Cat
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法： 货号 名称
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BioLegend 480131 MojoSort™ Human CD3 T Cell Isolation Kit
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