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- 2025年11月05日
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99
- 英文名：
BioLegend 439807 LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 439807 LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit
产品简介：
产品品牌：BioLegend
产品货号：439807
产品名称：BioLegend 439807 LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit
产品规格：1 Pre-coated Plate
产品说明：
Regulatory Status RUO
Other Names OVA-IgE Precoated ELISA Kit
描述
免疫球蛋白E长期以来一直被认为是特应性疾病的主要分子成分，包括过敏和哮喘。哮喘是一种慢性呼吸道疾病，其特征是支气管炎症和气道高反应性。临床研究发现哮喘与血清IgE水平密切相关。已在过敏患者的鼻黏膜中检测到过敏原特异性 IgE。对人类过敏性哮喘小鼠模型的检查为将气道炎症与气道功能障碍联系起来的病理生物学事件提供了新的见解。
卵清蛋白（OVA）是蛋清中的主要蛋白质，占总蛋白质的60-65%。卵清蛋白是几个不同研究领域的重要蛋白质，通常用于刺激小鼠的过敏反应（例如，建立气道高反应性的模型过敏原）。
BioLegend LEGEND MAX™小鼠OVA特异性IgE ELISA试剂盒是一种夹心酶联免疫吸附测定（ELISA），带有预涂有OVA的96孔试板。该试剂盒专为准确定量来自细胞培养上清液、血清、血浆和其他生物体液的小鼠 OVA 特异性 IgE 而设计。它通过即用型试剂进行了分析验证。
Immunoglobulin E has long been regarded as a major molecular component of atopic diseases, including allergy and asthma. Asthma is a chronic respiratory disease characterized by bronchial inflammation and airway hyperresponsiveness. Clinical studies have found a close association between asthma and serum IgE levels. Allergen-specific IgE has been detected in nasal mucosa from allergic patient. Examination of murine models of human allergic asthma has provided novel insight into the pathobiologic events linking airway inflammation with airway dysfunction.
Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice (e.g. established model allergen for airway hyperresponsiveness).
The BioLegend LEGEND MAX™ Mouse OVA specific IgE ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with OVA. This kit is specifically designed for the accurate quantitation of mouse OVA specific IgE from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
Product Details
Verified Reactivity Mouse
Application ELISA
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文献和实验should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Production of Monoclonal Antibody by DNA Immunization with Electroporation
construct was injected intramuscularly with in vivo electroporation into mice, and the specific antibody production in mice was analyzed by enzyme-linked immunosorbent assay (ELISA). Hybridomas were generated by fusing mouse splenocytes with myeloma cells
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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