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- BioLegend
- 420801
- BioLegend
- 2025年11月04日
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- 库存：
99
- 英文名：
BioLegend 420801 Fixation Buffer
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 420801 Fixation Buffer
产品简介：
产品品牌：BioLegend
产品货号：420801
产品名称：BioLegend 420801 Fixation Buffer
产品规格：100 mL
产品说明：
Regulatory Status RUO
Other Names Fixative, *
描述
固定缓冲液可用于细胞内染色程序，例如，用于制备用于染色细胞内细胞因子或其他蛋白质的细胞。固定缓冲液用于在透化之前使用透化洗涤缓冲液（Cat.第421002号）。BioLegend的固定缓冲液采用低背景的预筛选多聚jia醛配制而成，因此产生最大的信噪比。
Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened * with low background, thus producing the greatest signal to noise ratio.
Product Details
Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application ICFC - Quality tested
ICC
Recommended Usage
For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains *, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Application Notes
This 1X PBS solution contains 4% *, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.
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文献和实验Methanol Fixation for Immunofluorescence
. We recommend that you try both types of fixation for your particular antibody/fusion protein when working out your initial immunofluorescence conditions. MeS Buffer 100 mM MeS, pH 6.9 1 mM EGTA 1 mM MgCl2 Store at 4°C Methanol Fix (100
Pouring Linear and Buffer-Gradient Sequencing Gels
to be read from a single run) up to 100 cm in length can be poured, as can wider gels (to enable more clones to be loaded onto a single gel). However, these larger gels are more difficult to handle after electrophoresis, i.e., during fixation, drying
tightly, and warm in a water bath. 5. Cytoskeleton buffer with 0.5 mg/ml NaBH4 , 30 ml. Prepare fresh before use in a fixation box. Bubbles should be visible. Store NaBH4 desiccated in a tightly-sealed container. 6. Phosphate buffered saline
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