BioLegend 426311 Western-Ready™ Protein Sample Loading Buffer (5X) 产品简介: 产品品牌:BioLegend 产品货号:426311 产品名称:BioLegend 426311 Western-Ready™ Protein Sample Loading Buffer (5X) 产品规格:20 mL
产品说明: 描述 蛋白质免疫印迹™蛋白样品上样缓冲液(5X)可用于制备非还原和还原SDS-PAGE样品。 Western-Ready™ Protein Sample Loading Buffer (5X) can be used for the preparation of samples for non-reducing and reducing SDS-PAGE.
Product Details Formulation 300 mM Tris-HCl pH 6.8, 10% Sodium dodecyl sulfate, 30% glycerol, 0.02% Bromophenol blue
Storage & Handling Ambient room temperature Application WB - Quality tested Recommended Usage Dilute Western-Ready™ Protein Sample Loading Buffer (5X) to a 1X concentration (1:4 by volume) using appropriate amount of sample and diluting solution (ie: water, lysis buffer, etc). For sample that are to be used for reducing PAGE, a reducing agent such as as β-ME, DTT, or TCEP must be added to buffer prior to mixing and heating sample.
Application Notes Western-Ready™ Protein Sample Loading Buffer (5X) contains SDS, which may precipitate at cooler temperatures. If preceipitates are observed in the buffer, heat in 37°C water bath for 5 min to bring SDS back into solution.