BioLegend 364116 PE anti-BrdU 产品简介: 产品品牌:BioLegend 产品货号:364116 产品名称:BioLegend 364116 PE anti-BrdU 产品规格:100 tests
产品说明: Clone 3D4 (See other available formats) Regulatory Status RUO Other Names 5-bromodeoxyuridine, bromodeoxyuridine Isotype Mouse IgG1, κ
描述 BrdU是尿苷衍生物和胸苷的结构类似物,可在细胞周期的S期掺入DNA中作为胸苷的替代品。可以用BrdU对细胞进行脉冲标记,并用针对BrdU的抗体进行分析,以确定在给定间隔内细胞周期S期的细胞比例。 BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
Product Details Antibody Type Monoclonal Host Species Mouse Immunogen Iodouridine-conjugated ovalbumin Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICFC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application Notes Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.
Antigen Details Structure Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.
Function The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area Cell Biology, Cell Cycle/DNA Replication, Immunology Molecular Family Nuclear Markers