BioLegend 322906 PE anti-human TSLPR (TSLP-R) 产品简介: 产品品牌:BioLegend 产品货号:322906 产品名称:BioLegend 322906 PE anti-human TSLPR (TSLP-R) 产品规格:100 tests
产品说明: Clone 1D3 (See other available formats) Regulatory Status RUO Other Names Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R Isotype Mouse IgG2a, λ
描述 TSLP-R又称胸腺基质淋巴生成素受体、细胞因子受体样2、CRL2和IL-XR,是一种I型膜受体,与IL-7R形成功能性异二聚体复合物以结合TSLP。TSLP-R包含正确蛋白质折叠所需的WSXWS基序和与JAK结合很重要的box1基序。TSLP-R的预测分子量约为40 kD,并且已经报道了通过交替剪接产生的两种亚型。TSLP-R在骨髓细胞(包括树突状细胞和活化的单核细胞)中优先表达,在T细胞中表达较弱。在心脏、骨骼肌和肾脏组织中也有表达的报道。TSLP与异二聚体功能受体(TSLP-R和IL-7R)结合激活JAK2,STAT3和STAT5以刺激细胞增殖。配体受体相互作用与过敏性疾病中造血系统的发展、树突状细胞成熟以及人Th2记忆T细胞的维持和极化有关。 TSLP-R, also known as thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind TSLP. The TSLP-R contains a WSXWS motif required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight approximately 40 kD, and two isoforms have been reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells including dendritic cells and activated monocytes, and is weakly expressed in T cells. Expression has also been reported in heart, skeletal muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Ligand receptor interactions haves been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergic diseases.
Product Details Verified Reactivity Human Antibody Type Monoclonal Host Species Mouse Immunogen Human TSLP-R:Fc protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application Notes For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 322905/322906) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)).
Antigen Details Structure Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs. Predicted molecular weight approximately 4 Distribution Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal muscle, and kidney
Function TSLP binding to functional receptor activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Has been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T
Interaction Together with IL-7R binds TSLP Cell Type Dendritic cells, Monocytes, T cells, Tregs Biology Area Immunology Molecular Family Cytokine/Chemokine Receptors 以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 159811 Spark Violet™ 423 anti-mouse CD19 Recombinant QA17A27 25 ug BioLegend 159812 Spark Violet™ 423 anti-mouse CD19 Recombinant QA17A27 100 ug BioLegend 160004 PE anti-mouse CD287 (TLR7) A94B10 100 ug BioLegend 160102 Purified anti-mouse CD192 (CCR2) Recombinant QA18A56 500 ug BioLegend 160203 PE anti-mouse CD31 (PECAM-1) W18222B 25 ug BioLegend 160204 PE anti-mouse CD31 (PECAM-1) W18222B 100 ug BioLegend 160303 Alexa Fluor® 647 anti-mouse CD45 S18009D 25 ug BioLegend 160306 Alexa Fluor® 488 anti-mouse CD45 S18009D 100 ug BioLegend 160905 PE anti-mouse Ly-6A/E (Sca-1) W18174A 25 ug BioLegend 161103 PE/Fire™ 640 anti-mouse CD11c Recombinant QA18A72 25 ug BioLegend 161203 PE anti-mouse CD62L W18021D 25 ug BioLegend 162105 APC anti-mouse CD25 Recombinant QA19A49 25 ug BioLegend 162703 PE anti-mouse AhR W16012C 25 ug BioLegend 163302 Purified anti-mouse Siglec-G SH1 100 ug BioLegend 163504 PE anti-mouse IFN-γ W18272D 100 ug BioLegend 163607 PE/Cyanine7 anti-mouse/human CD44 Recombinant QA19A43 25 ug BioLegend 164004 PE anti-mouse CD13 Recombinant QA19A79 100 ug BioLegend 200205 PE anti-rat CD80 3H5 50 ug BioLegend 200303 Biotin anti-rat CD86 24F 50 ug BioLegend 200307 PE anti-rat CD86 24F 50 ug BioLegend 200308 PE anti-rat CD86 24F 200 ug BioLegend 200403 PE anti-rat CD106 MR106 50 ug