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99
- 英文名：
BioLegend 304029 Pacific Blue™ anti-human CD45
- 供应商：
北京云肽生物科技有限公司
BioLegend 304029 Pacific Blue™ anti-human CD45
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 304029 Pacific Blue™ anti-human CD45
产品简介：
产品品牌：BioLegend
产品货号：304029
产品名称：BioLegend 304029 Pacific Blue™ anti-human CD45
产品规格：100 tests
产品说明：
Clone HI30 (See other available formats)
Regulatory Status RUO
Workshop IV N816
Other Names LCA, T200
Isotype Mouse IgG1, κ
描述
CD45 是一种 180-240 kD 单链 I 型膜糖蛋白，也称为白细胞共同抗原 （LCA） 和 T200。它是一种酪氨酸磷酸酶，表达在所有造血细胞的质膜上，红细胞和血小板除外。CD45 是一种信号分子，可调节多种细胞过程，包括细胞生长、分化、细胞周期和致癌转化。CD45 通过去磷酸化底物（包括 p56Lck、p59Fyn 和其他 Src 家族激酶）在 T 细胞和 B 细胞抗原受体介导的活化中起关键作用。CD45 与 T 和 B 淋巴细胞上的淋巴细胞磷酸酶相关磷蛋白 （LPAP） 非共价相关。据报道，CD45 可结合半乳糖凝集素-1 并与其他几种细胞表面抗原（包括 CD1、CD2、CD3 和 CD4）相关。
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product Details
Isotype Control Pacific Blue™ Mouse IgG1, κ Isotype Ctrl
Verified Reactivity Human
Reported Reactivity Chimpanzee
Antibody Type Monoclonal
Host Species Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licensesExcitation LaserViolet Laser (405 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of *-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Antigen Details
Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
Function TCR and BCR mediated activation
Ligand/Receptor Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family CD Molecules
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
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BioLegend 144502 Purified anti-mouse CD193 (CCR3) J073E5 500 ug
BioLegend 144506 PE anti-mouse CD193 (CCR3) J073E5 100 ug
BioLegend 144510 FITC anti-mouse CD193 (CCR3) J073E5 100 ug
BioLegend 144511 APC anti-mouse CD193 (CCR3) J073E5 25 ug
BioLegend 144516 PerCP/Cyanine5.5 anti-mouse CD193 (CCR3) J073E5 100 ug
BioLegend 144601 Purified anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 50 ug
BioLegend 144602 Purified anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 500 ug
BioLegend 144603 FITC anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 50 ug
BioLegend 144604 FITC anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 500 ug
BioLegend 144605 Alexa Fluor® 647 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 25 ug
BioLegend 144606 Alexa Fluor® 647 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
BioLegend 144608 PE anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
BioLegend 144609 PerCP/Cyanine5.5 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 25 ug
BioLegend 144610 PerCP/Cyanine5.5 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
BioLegend 144612 Alexa Fluor® 488 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
BioLegend 144613 Pacific Blue™ anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 25 ug
BioLegend 144614 Pacific Blue™ anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
BioLegend 144617 APC anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 25 ug
BioLegend 144618 APC anti-MU/HU GL7 Antigen (T/B Cell Act. Marker) GL7 100 ug
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BioLegend 304029 Pacific Blue™ anti-human CD45
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