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- 文献和实验
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- 库存:
99
- 英文名:
Flex-T™ Biotin H-2 K(b) TRP2 Monomer (SVYDFFVWL)
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国 BioLegend 公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长,但它聚集了一批来自 PharMingen 公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 280061 Flex-T™ Biotin H-2 K(b) TRP2 Monomer (SVYDFFVWL)
产品简介:
产品品牌:BioLegend
产品货号:280061
产品名称:BioLegend 280061 Flex-T™ Biotin H-2 K(b) TRP2 Monomer (SVYDFFVWL)
产品规格:25 µg
产品说明:
描述
CD8 + T细胞是免疫系统的重要组成部分。它们通过对I 类 MHC(人类中的 HLA)分子呈递的抗原(肽表位)的 TCR 识别来发挥作用。 因此,同源MHC/肽复合物的TCR识别可用于检测抗原特异性CD8 + T细胞。
SVYDFFVWL肽来自酪氨酸酶相关蛋白2(TRP2,180-188)。
CD8+ T cells are essential components of the immune system. They function through TCR recognition of antigens (peptide epitopes) presented by the class I MHC (HLA in humans) molecules. Thus, TCR recognition of cognate MHC/peptide complex can be utilized for the detection of antigen-specific CD8+ T cells.
The SVYDFFVWL peptide is from Tyrosinase Related Protein2 (TRP2, 180-188).
Product Details
Verified Reactivity Mouse
Formulation
PBS containing 0.5% BSA and protease inhibitors
Concentration 0.1 mg/mL
Storage & Handling
Store frozen at -20°C or -80°C. Aliquot upon receipt before freezing; avoid repeated freeze-thaw cycles. Protect from light.
Application ELISA - Quality tested
FC - Verified
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 30 μL of 0.1 mg/mL biotinylated monomer, incubated with desired streptavidin fluorophore conjugate, following our recommended protocol. This will generate tetramers sufficient for 15 tests.
Application Notes
Two-color staining with the same allele/peptide combination is recommended for flow cytometry analysis to increase the resolution and specificity of the assay.
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文献和实验to avidin magnetic beads (Dynal™). The final elutes, following the binding of the biotin linked RNAs with the avidin magnetic beads and washing, were then DNAse, RNAse H or RNAse A treated, RT (BioRad™ iScript) reacted and PCR amplified. References 1.Kim
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
‐1037. Loewer, S., Cabili, M.N., Guttman, M., Loh, Y.H., Thomas, K., Park, I.H., Garber, M., Curran, M., Onder, T., Agarwal, S., Manos, P.D., Datta, S., Lander, E.S
secondary antibody review -- data from 99 publications
ref host class subclass conjugation experiment dilution detail supplier 1 goat Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat Alexa Fluor 546 immunocytochemistry 1:250
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