产品说明: Clone 10E5 (See other available formats) Regulatory Status RUO Other Names DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1 Isotype Rat IgG2b, κ
描述 CD226(DNAM-1)在天然CD8+细胞、CD4+T细胞、巨噬细胞和NK细胞上组成性表达。据报道,这种抗体(10E5)与约40%的失活CD4+细胞结合,仅与分化的Th1细胞结合,而不与Th2或Th0细胞结合。还报道,它抑制抗原特异性T细胞扩增和Th1细胞介导的EAE(实验性过敏性脑炎)。 CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on CD4+ T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4+ cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.
Product Details Isotype Control Purified Rat IgG2b, κ Isotype Ctrl Verified Reactivity Mouse Antibody Type Monoclonal Host Species Rat Immunogen Th1 polarized T cell clones Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Application FC - Quality tested IHC-F - Verified
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details Structure 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains. Distribution Expressed on majority of CD4 and CD8 T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.++
Function Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation Ligand/Receptor CD112 and CD155 Cell Type B cells, Macrophages, Monocytes, NK cells, T cells, Th1 Biology Area Immunology Molecular Family Adhesion Molecules, CD Molecules
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 109837 Brilliant Violet 510™ anti-mouse CD45.2 104 125 uL BioLegend 109838 Brilliant Violet 510™ anti-mouse CD45.2 104 50 ug BioLegend 109841 Brilliant Violet 605™ anti-mouse CD45.2 104 50 ug BioLegend 109850 Alexa Fluor® 594 anti-mouse CD45.2 104 100 ug BioLegend 109905 FITC anti-mouse I-Ak (Aβk) 10-3.6 50 ug BioLegend 109908 PE anti-mouse I-Ak (Aβk) 10-3.6 200 ug BioLegend 110608 PE anti-mouse H-2Dd 12462 200 ug BioLegend 110702 Purified anti-mouse CD45.1 A20 500 ug BioLegend 110704 Biotin anti-mouse CD45.1 A20 500 ug BioLegend 110705 FITC anti-mouse CD45.1 A20 50 ug BioLegend 110706 FITC anti-mouse CD45.1 A20 500 ug BioLegend 110707 PE anti-mouse CD45.1 A20 50 ug BioLegend 110708 PE anti-mouse CD45.1 A20 200 ug BioLegend 110713 APC anti-mouse CD45.1 A20 25 ug BioLegend 110714 APC anti-mouse CD45.1 A20 100 ug BioLegend 110715 APC/Cyanine7 anti-mouse CD45.1 A20 25 ug BioLegend 110716 APC/Cyanine7 anti-mouse CD45.1 A20 100 ug BioLegend 110720 Alexa Fluor® 647 anti-mouse CD45.1 A20 100 ug BioLegend 110722 Pacific Blue™ anti-mouse CD45.1 A20 100 ug BioLegend 110723 Alexa Fluor® 700 anti-mouse CD45.1 A20 25 ug BioLegend 110724 Alexa Fluor® 700 anti-mouse CD45.1 A20 100 ug BioLegend 110725 PerCP anti-mouse CD45.1 A20 25 ug