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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
Pierce Protein-Free T20 (TBS) Blocking Buffer
- 供应商:
北京云肽生物科技有限公司
我们北京云肽生物科技有限公司作为Thermo Scientific公司的特约经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
赛默飞世尔科技公司(Thermo Fisher Scientific)赛默飞世尔科技是全球科学服务领域的lingdaozhe(纽约证交所代码:TMO),致力于帮助客户使世界更健康,更清洁,更安全。
公司借助于Thermo Scientific、Life Technologies、Fisher Scientific和Unity™ Lab Services四个首要品牌,帮助客户解决在分析化学领域从常规的测试到复杂的研发项目中所遇到的各种挑战。
Thermo Scientific能够为客户提供一整套包括高端分析仪器、实验室装备、软件、服务、耗材和试剂在内的实验室综合解决方案。Thermo Fisher Scientific将努力为客户提供jiwei便 捷的采购方案,为科研的飞速发展不断地改进工艺技术,提升客户价值。
Thermo Scientific37571Pierce Protein-Free T20 (TBS) Blocking Buffer/T20(TBS)封闭缓冲液/封闭液,产品简介:
产品品牌:Thermo Scientific
产品货号:37517
产品名称:SuperBlock (PBS) Blocking Buffer - Blotting/PBS 封闭缓冲液/封闭液
产品规格:1 L
Thermo Scientific37571Pierce Protein-Free T20 (TBS) Blocking Buffer/T20(TBS)封闭缓冲液/封闭液,产品说明:
Thermo Scientific SuperBlock 印迹封闭缓冲液是经优化的 PBS 溶液,其中含有的一种蛋白可为 Western 印迹分析提供极其快速有效的封闭。使用 SuperBlock 缓冲液封闭微孔板、膜或组织,可确保大多数检测体系实现高信噪比。
SuperBlock 印迹封闭缓冲液的特点包括:
• 快速—可在5至10分钟内封闭膜
• 灵活—保证不含生物素,可用于链霉素亲和素系统
• 低背景—非血清蛋白溶液可实现高信噪比
• 稳定—缓冲液可在 4°C 下储存一年;封闭的培养板在干燥状态下可储存长达12个月
使用 SuperBlock 缓冲液封闭微孔板、膜或组织,可确保大多数检测系统实现高信噪比。基于蛋白质的制剂,不含任何免疫球蛋白、白蛋白或内源性生物素,兼容许多传统封闭液失效的情形。此缓冲液特别适合封闭和稳定包被聚苯乙烯微孔板(96 孔板),以便干燥和储存以备日后使用。
Thermo Scientific37571Pierce Protein-Free T20 (TBS) Blocking Buffer/T20(TBS)封闭缓冲液/封闭液,产品订购信息:
品牌 货号 产品名称 规格
Thermo Scientific 37571 Pierce Protein-Free T20 (TBS) Blocking Buffer/T20(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37573 Pierce Protein-Free T20 (PBS) Blocking buffer/T20(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37570 Pierce Protein-Free (TBS) Blocking Buffer/无蛋白(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37585 Pierce Protein-Free (TBS) Blocking Buffer/无蛋白(TBS)封闭缓冲液/封闭液 100 mL
Thermo Scientific 37572 Pierce Protein-Free (PBS) Blocking buffer/无蛋白(PBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37584 Pierce Protein-Free (PBS) Blocking buffer/无蛋白(PBS)封闭缓冲液/封闭液 100 mL
Thermo Scientific 37575 Pierce Fast Blocking Buffer/快速封闭缓冲液/封闭液 500 mL
Thermo Scientific 37576 Pierce Fast Blocking Buffer/快速封闭缓冲液/封闭液 100 mL
Thermo Scientific 37545 SuperBlock (TBS) Blocking Buffer Dry Blend SuperBlock /封闭液 5 packs
Thermo Scientific 37520 Blocker BSA (10X) in TBS / Blocker BSA(10X)TBS封闭缓冲液/封闭液 125 mL
Thermo Scientific 37525 Blocker BSA in PBS (10X)/Blocker(10X) BSA封闭缓冲液/封闭液 100 mL
Thermo Scientific 37528 Blocker Casein in PBS/酪蛋白封闭缓冲液/封闭液 1 L
Thermo Scientific 37582 Blocker Casein in PBS/酪蛋白封闭缓冲液/封闭液 100 mL
Thermo Scientific 37532 Blocker Casein in TBS/酪蛋白封闭缓冲液/封闭液 1 L
Thermo Scientific 37583 Blocker Casein in TBS/酪蛋白封闭缓冲液/封闭液 100 mL
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文献和实验[分享] Strip Antibodies and Re-use Protein blots membrane
10min in PBST 5. Transfer the membrane to strip buffer (preheated to 55-65C) for 20min, 6. Repeat step 5 once 7. Rinse 3X in PBST followed by 10min in PBST 8. Check strip efficiency (normally, it is OK, but if the expression is too high
Western Blotting and Immunostaining Protocol
Wash the membrane with 3 x 15 ml of TBS. Discard washes. Add 15 mls of secondary antibody diluted in blocking buffer (use 1 drop per 15 mls) to the membrane and incubate for 1 hour with gentle agitation. Discard secondary antibody solution.
Western Blotting and Immunostaining
and add 15 mls of primary antibody solution diluted as appropriate in fresh blocking buffer. Incubate for 1 hour. Filter (0.22μm) the primary antibody solution into a 50ml tube, and store at 4℃ for next use. Wash the membrane with 3 x 15 ml of TBS
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