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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
1年
- 英文名:
Anti-Siglec-15 / CD33L3 Reference Antibody (AB-25E9)
- 库存:
999
- 供应商:
百生跃(Bioleaper)
- 规格:
50μg
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
by feeding the others (and collecting supernatants for testing) - again remember that most hybridomas will be ADHERENT, so will need vigorous pipetting to get them into suspension for splitting. h) Split wells into 25ml flasks to make 2 x 2 freezes
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
below. Blocking step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites
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