This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Rat COL3(Collagen Type Ⅲ) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Rat COL3(Collagen Type Ⅲ) antibody. After incubation, add Biotin-conjugated anti-Rat COL3(Collagen Type Ⅲ) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Rat COL3(Collagen Type Ⅲ) in the sample was proportional to the OD value. The concentration of Rat COL3(Collagen Type Ⅲ) in the sample can be calculated by drawing a standard curve.
灵敏度
37.5 pg/mL
Sensitivity
37.5 pg/mL
检测范围
62.5-4000 pg/mL
Examination range
62.5-4000 pg/mL
特异性
可检测样本中的大鼠Ⅲ型胶原,且与其类似物无明显交叉反应。
Specificity
This kit recognizes Rat COL3(Collagen Type Ⅲ) in samples. No significant cross-reactivity or interference between Rat COL3(Collagen Type Ⅲ) and analogues was ob/served.
Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率
分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Recovery
A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.