Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
别名
LCN2; Lipocalin 2; Oncogene 24p3; MSFI
相关类别
ELISA
储存
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应用
用于人血清、血浆或其他相关生物液体中中性粒细胞明胶酶相关脂质运载蛋白的测定。 For the determination of Human NGAL(Neutrophil Gelatinase Associated Lipocalin) concentrations in serum, plasma and other related biological fluids.
This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human NGAL(Neutrophil Gelatinase Associated Lipocalin) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human NGAL(Neutrophil Gelatinase Associated Lipocalin) antibody. After incubation, add Biotin-conjugated anti-Human NGAL(Neutrophil Gelatinase Associated Lipocalin) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human NGAL(Neutrophil Gelatinase Associated Lipocalin) in the sample was proportional to the OD value. The concentration of Human NGAL(Neutrophil Gelatinase Associated Lipocalin) in the sample can be calculated by drawing a standard curve.
灵敏度
18.75 pg/mL
Sensitivity
18.75 pg/mL
检测范围
31.25~2000 pg/mL
Examination range
31.25~2000 pg/mL
特异性
可检测样本中的人中性粒细胞明胶酶相关脂质运载蛋白,且与其类似物无明显交叉反应。
Specificity
This kit recognizes Human NGAL(Neutrophil Gelatinase Associated Lipocalin) in samples. No significant cross-reactivity between Human NGAL(Neutrophil Gelatinase Associated Lipocalin) and analogues was ob/served.
Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率
分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Recovery
A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.