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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
CD33 antigen-like 3; SIGLEC-15; CD33L3; sialic acid-binding Ig-like lectin 15; Siglec15
- 抗体英文名:
CD33 antigen-like 3; SIGLEC-15; CD33L3; sialic acid-binding Ig-like lectin 15; Siglec15
- 靶点:
-
- 浓度:
>95% by SDS-PAGE
- 应用范围:
-
- 宿主:
Human
- 适应物种:
-
- 保质期:
Within 1 week, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.
- 抗原来源:
-
- 目录编号:
-
- 级别:
-
- 库存:
现货
- 供应商:
爱必信(上海)生物科技有限公司
- 标记物:
-
- 克隆性:
-
- 保存条件:
Within 1 week, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.
- 形态:
Lyophilized Powder
- 亚型:
-
- 免疫原:
-
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥2800.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥5300.0 |
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文献和实验Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
function. Many R proteins are associated with the plasma membrane (PM) and expressed at low levels. Here, we describe a method to purify such low-abundance PM R protein �complexes from Arabidopsis using a biotinylated affinity tag, called the HPB tag
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
。结果如图3所示,PA tag附加到任何一个位置上都可以很好地将目的蛋白进行纯化。图3为分泌蛋白的纯化结果,而我们对膜蛋白质和细胞内蛋白的纯化也已经取得成功,可以纯化分子量约15kDa到500kDa的蛋白。但是使用PA肽洗脱时,每一个阶段都要进行5分钟的孵育。否则很多组分中的蛋白会变得分散。这是由于NZ-1和PA tag的分离太慢造成的,而PA肽和PA tag的竞争结合也需要一定的时间。另外,除了纯化外还可以将标签用于检测,使PA tag有更高的实用性。使用免疫学的方法在PA tag的帮助下可以更快更
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