Sino Biological 重组人THRB蛋白(His标签) 高纯度 大肠杆菌表达

纯度
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the Human THRB (P10828-1) (Glu202-Asp461) was expressed, with a polyhistidine tag at the N-terminus.
表达宿主
E.coli
种属
Human
预测 N 端
Met
分子量
The recombinant Human THRB consists of 267 amino acids and predicts a molecular mass of 30.33 kDa.
缓冲液
Lyophilized from sterile 50mM Tris, 1mm Tcep, pH 8.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.




THRB Protein, Human, Recombinant (His Tag), HPLC-verified: 别称
C-ERBA-2 Protein, Human; C-ERBA-BETA Protein, Human; ERBA2 Protein, Human; GRTH Protein, Human; NR1A2 Protein, Human; PRTH Protein, Human; THR1 Protein, Human; THRB1 Protein, Human; THRB2 Protein, Human
THRB 背景信息
Thyroid hormone receptor beta (THRB), also known as TRbeta and NR1A2, belongs to the nuclear hormone receptor family. Featuring an N-terminal transactivation domain, a central DNA-binding domain, and a hormone/ligand-binding domain, THRB isoform 1/2 expression localizes predominately to the liver and pituitary, respectively. THRB regulates the feedback of T3-dependent thyrotropin-stimulating hormone (TSH) transcription in the pituitary by binding to thyroid hormone (TH) response elements present on promoters of TH-responsive genes. Located on chromosome 3p24, THRB gene mutation has been linked to autosomal dominant resistance to TH (RTH), which is characterized by goiter and elevated levels of T3, T4, and TSH. THRB is a known pharmacological target for the treatment of non-alcoholic steatohepatitis (NASH). THRB may be a target for future cancer therapies since its knockdown enhanced the viability of endometrial cancer cells and activated the mTOR-4EBP1/eIF4G pathway.
全称
thyroid hormone receptor, beta