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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human PHD finger protein 6 (PHF6)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human PHD finger protein 6 (PHF6)品名简称:
Recombinant Human PHF6 protein货号:
CSB-EP017917HUa0规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PHF6Uniprot No.:
Q8IWS0别名:
(PHD-like zinc finger protein)种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
39.2 kDa表达区域:
2-312aa氨基酸序列:
SSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMAYCRKHKKTAHNSEAADLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRPKCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMVCSFYICYATLHLICCFKFRVHPKFIQSSENLK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.功能2:
/文献:
"PHF6 regulates cell cycle progression by suppressing ribosomal RNA synthesis." Wang J., Leung J.W., Gong Z., Feng L., Shi X., Chen J. J. Biol. Chem. 288:3174-3183(2013)研究领域:
Epigenetics and Nuclear Signaling请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Generation and Functional Analysis of Zinc Finger Nucleases
of concept, showing that inserting a DSB in the target locus leads to gene correction frequencies of 1–18% in human cells. In this paper, we describe how zinc finger nucleases are assembled by polymerase chain reaction (PCR) and present two methods to assess
这类蛋白因子往往含有几个相同的指结构, 每一个指结构具有以下的保守序列: Cys-X2-4-Cys-Phe-X5-Leu-X2-His-X3-His。这段保守序列中的两个半胱氨酸残基和两个组氨酸残基与锌离子形成配位键, 形成环, 因此称为锌指, 上述具有Cys和His的指结构称为Cys2/His2指。每个指结构有23个氨基酸残基, 指结构之间的距离为7~8个氨基酸残基。 另一类指结构是Cys2/Cys2的锌指结构, 指结构中保守序列是:Cys-X2-Cys-X13
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
Wiley & Sons, Inc. Keywords: pronucleus injection; gene targeting; mouse mutant; zinc?finger nuclease; TAL nuclease; homologous recombination
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