N/A蛋白|卡努斯马莫雷斯N/A蛋白|卡努斯马莫雷斯N/A重

Research Areas

Others

Uniprot ID

Q26443

Gene Names

N/A

Alternative Name(s)

(CGX-1002)

Lead Time

13-23 business days

Organism

Conus marmoreus (Marble cone)

Source

E.coli

Expression Region

52-82aa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Target Protein Sequence

ACSKKWEYCIVPILGFVYCCPGLICGPFVCV

MW

30.3 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

Not test.

Biological_Activity

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Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance

MuO-conotoxins are gating-modifier toxins that inhibit sodium current by trapping the domain II voltage sensor in the closed position to prevent opening of the sodium channel. This toxin has a preference for Nav1.4/SCN4A over Nav1.2/SCN2A sodium channels. It blocks Nav channels by interacting mainly with the C-terminal part of the pore loop of domain-3. It also blocks fast-inactivating calcium current. Blocks Nav1.8/SCN10A sodium channels and has potent and long-lasting local anesthetic effects. It can also block propagation of action potentials in A- and C-fibers in sciatic nerve as well as skeletal muscle in isolated preparations.

Function

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