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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Aequorea victoria Aequorin-1
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
BiochemicalsUniprot ID
P07164Gene Names
/Alternative Name(s)
Lead Time
3-7 business daysOrganism
Aequorea victoria (Water jellyfish) (Mesonema victoria)Source
E.coliExpression Region
8-196aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal 6xHis-taggedTarget Protein Sequence
VKLTPDFDNPKWIGRHKHMFNFLDVNHNGRISLDEMVYKASDIVINNLGATPEQAKRHKDAVEAFFGGAGMKYGVETEWPEYIEGWKRLASEELKRYSKNQITLIRLWGDALFDIIDKDQNGAISLDEWKAYTKSDGIIQSSEDCEETFRVCDIDESGQLDVDEMTRQHLGFWYTMDPACEKLYGGAVPMW
25.8 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验一、 GFP背景概述 绿色荧光蛋白(green fluorescent protein, GFP)最早由Osamu Shimomura于1962年在水母(Aequorea victoria)(图2a)中发现。这种蛋白质在蓝色波长范围的光照激发下发出绿色荧光,其发光过程需要冷光蛋白质Aequorin的帮助,而且,这个冷光蛋白质可与钙离子(Ca2+)相互作用(图3)。在Aequorea victoria中发现的野生型绿色荧光蛋白的分子量较小,仅为27~30kDa,而编码GFP的基因序列也很短
GFP Fusion Proteins to Study Signaling in Live Cells
In just a few years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has jumped from relative obscurity to become one of the most widely studied and exploited proteins in cell biology. Discovered by Shimomura et al
Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
fluorescent protein (GFP) from the jellyfish Aequorea victoria has been enormously advantageous. As polypeptides, aequorin and GFP allow their endogenous production in cell systems as diverse as bacteria, yeast, slime molds, plants, and mammalian cells
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