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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli O157:H7 Major curlin subunit (csgA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli O157:H7 Major curlin subunit (csgA)品名简称:
Recombinant E.coli O157:H7 csgA protein货号:
CSB-YP821944EOD规格:
20μg价格:
2616LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
csgAUniprot No.:
Q93U24别名:
/种属:
Escherichia coli O157:H7蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
14.6 kDa表达区域:
21-152aa氨基酸序列:
GVVPQYGGGGGNHGGGGNNSGPNSELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Curlin is the structural subunit of the curli. Curli are coiled surface structures that assemble preferentially at growth temperatures below 37 degrees Celsius. Curli can bind to fibronectin.功能2:
/文献:
"Mutations in the csgD promoter associated with variations in curli expression in certain strains of Escherichia coli O157:H7." Uhlich G.A., Keen J.E., Elder R.O. Appl. Environ. Microbiol. 67:2367-2370(2001)研究领域:
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文献和实验摘要 基因重组蛋白在大肠杆菌中表达时,由于表达量高,往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率,是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述,为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止,人们表达的重组蛋白质已有4000多种,其中用E.coli表达的蛋白质要占90%以上,尽管基因重组技术为大规模生产目标蛋白质提供
关于论坛中所有Native PAGE/非变性电泳及蛋白回收的经典总结
形成3个一圈的螺旋)可能是使鞭毛状腺丝蛋白成为弹性最好的蜘蛛丝(>200% 伸缩性)的重要原因。 本实验室参考了家蚕丝心蛋白基因中密码子的偏爱, 合成了一个编码了包含有多聚丙氨酸(A)n序列, (GPGXX)序列和(Gly-Gly-X, X为Ala, Gln和Thr)序列的321 bp仿蜘蛛牵丝的单体基因, 在E.coli中逐步加倍至约2400 bp后, 与GFP基因融合, 用电穿孔方法导入蚕卵, 通过“亮茧”表型, 基因鉴定(PCR, Southern 印迹), 及茧壳中GFP的ELISA阳性
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
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