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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Arabidopsis thaliana IAA6 protein (46-91aa)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Arabidopsis thaliana
- 保质期:
6个月
- 抗原来源:
Arabidopsis thaliana (Mouse-ear cress)
- 目录编号:
Q38824
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
/
- 应用范围:
ELISA, WB
- 浓度:
>95%,Affinity-chromatography
- 靶点:
IAA6
- 抗体英文名:
IAA6 Antibody
- 抗体名:
Auxin-responsive protein IAA6 (Indoleacetic acid-induced protein 6) IAA6 SHY1 At1g52830 F14G24.10
- 规格:
100μl/50μl/20μl
| 规格: | 100μl | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥880.0 |
| 规格: | 20μl | 产品价格: | ¥440.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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文献和实验by tank with regulator; at least 1000 psi pressure) Iodoacetamide (IAA) Modified trypsin (sequencing grade) (optional, see Step 7) Poroszyme-immobilized trypsin (Applied Biosystems) (Optional, see Step 7) Soluble protein fractions If proteins
Pull-Down Assays for Plant Hormone Research
up a selection of players that may or may not interact directly to carry the signal in question and further insight inevitably leads to some sort of biochemistry. Using the example of the auxin-regulated interaction between the auxin receptor TIR1 and the Aux/IAA
Fusion Protein Isolation 融合蛋白分离纯化
as above except NO tyrptophan,and place in a sterile 1 or 2 liter flask. 3.Shake until OD599nm=0.2-0.3.This will take between 1-4 hours. 4.Add indoleacrylic acid to 30 µg/ml (600µl of a 5 mg/ml stock made fresh in ethanol).The IAA must be fresh or the induction will be poor







