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- Agrisera
- 瑞典
- AS11 1749
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
IAA | Auxin ELISA quantitation kit
- 规格：
1kit
| Background | this ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The IAA-indole-3-acetic acid (C1') hormone specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples. |
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Reaction wells | antibody coated and blocked, 5pcs for 480 assays, 60 strips with 8 wells
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Tracer | 20 – 50 µl
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Tracer diluent | 5x 250 mM TBS Tris, 10 mM NaCl, 1 mM MgCl2, pH 7.5 stock + 0.02 % NaN3
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Reaction and wash solution | 10x TBS stock+0.02 % NaN3
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Stopping reagent | 2x 5 N KOH stock
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Substrate diluent | 10x 500 mM NaHCO3 stock, pH 9.6+0 0.02 %. 0 0.02 % NaN3
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Substrate | 100 mg
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Standards | 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol
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Assay development time | 4-5 hours
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Sensitivity | 0.01 to 10 pmol/50 µl
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Plant extract volume | 50 µl (for using on algae - please inquire)
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名称：IAA | Auxin ELISA quantitation kit
货号：AS11 1749
品牌：Agrisera
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文献和实验IAA Analysis in Transgenic Plants
-conjugates remains a vital part of the analysis if we are to understand auxin action. Several methods are available utilizing immunoassays, GC-MS, high-performance liquid chromatography (HPLC), and fluorescence. One reliable method for IAA analysis
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
植物吲哚乙酸（IAA） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定植物相关样本中吲哚乙酸（IAA）含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 植物吲哚乙酸（IAA ） 水平。用纯化的 植物吲哚乙酸（IAA ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 植物吲哚乙酸（IAA ） ，再与HRP 标记的 植物吲哚乙酸（IAA ） 抗体结合，形成抗体
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