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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
请将未开封使用的保存于2-14℃
- 保质期:
1年
- 英文名:
Pulldown™ Anti-DDDDK Magnetic IP Kit
- 库存:
100
- 供应商:
艾美捷科技
- 规格:
20T
Pulldown ™ Flag标签蛋白免疫沉淀试剂盒(磁珠法),Pulldown™ Anti-DDDDK Magnetic IP Kit,标签内参抗体,Pulldown ™ Flag标签蛋白免疫沉淀试剂盒(磁珠法),Pulldown™ Anti-DDDDK Magnetic IP Kit
产品名称:Pulldown ™ Flag标签蛋白免疫沉淀试剂盒(磁珠法)-Pulldown™ Anti-DDDDK Magnetic IP Kit
产品货号:D-AKE2014-20T
产品规格:20T
背景资料:抗DDDDK磁性IP套件
保存建议:Pulldown ™ Flag标签蛋白免疫沉淀试剂盒(磁珠法)-Pulldown™ Anti-DDDDK Magnetic IP Kit请将未开封使用的保存于2-14℃;启用后的试剂盒
产品描述:试剂盒包含高质量的Anti-Flag磁珠及经过优化验证的免疫沉淀必要试剂,使免疫沉淀(IP)实验更加简单、便捷、高效。
点击:Pulldown ™ Flag标签蛋白免疫沉淀试剂盒(磁珠法)-Pulldown™ Anti-DDDDK Magnetic IP Kit查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Biogradetech公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
RNAs;Following the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution
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