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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Human
96T
货号 | FEK0404 |
---|---|
产品名称 | Human IL-4 Quick ELISA Kit |
产品描述 | For quantitative detection of human IL-4 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, H25-S153 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1.5pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IL4 |
基因全名 | interleukin 4 |
Uniprot ID | IL4: P05112 |
指标别名 | B cell growth factor 1|B cell stimulatory factor 1|BCGF 1|BCGF1|Binetrakin|BSF 1|BSF1|IL 4|Interleukin 4|Lymphocyte stimulatory factor 1|MGC79402|Pitrakinra |
背景资料 | Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
FEK0404-CAP | Anti-human IL-4 Pre-coated 96-well Strip Microplate | 1 |
EK0404-ST | human IL-4 standard | 10ng/tube×2 |
FEK0404-H | HRP Linked Anti-human IL-4 Antibody | 6ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | S top solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 216 | 508 | 33 | 195 | 532 |
Standard deviation | 2.03 | 12.52 | 21.33 | 2.04 | 11.89 | 29.26 |
CV(%) | 5.8% | 5.8% | 4.2% | 6.2% | 6.1% | 5.5% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.040 | 0.148 | 0.238 | 0.516 | 0.755 | 1.314 | 2.094 | 2.570 |
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