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发货周期:现货
博士德生物
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Mouse
96T
货号 | FEK0919 |
---|---|
产品名称 | Mouse CXCL5 / LIX / ENA-78 / GCP 2 Quick ELISA Kit |
产品描述 | Mouse CXCL5 / LIX / ENA-78 / GCP 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 10pg/ml. |
免疫原 | Expression system for standard: E.coli; Immunogen sequence: V45-A118 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 15.6 pg/ml - 1,000 pg/ml |
基因名称 | Cxcl5 |
基因全名 | C-X-C motif chemokine 5 |
Uniprot ID | Cxcl5: P50228 |
背景资料 | C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
FEK0919-CAP | 96-well plate precoated with anti-Mouse Cxcl5 antibody | 1 |
EK0919-ST | lyophilized recombinant Mouse Cxcl5 standard | 10ng/tube×2 |
FEK0919-DA | biotinylated anti-Mouse Cxcl5 antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 35 | 166 | 388 | 36 | 150 | 360 |
Standard deviation | 2.17 | 8.13 | 16.93 | 2.48 | 8.1 | 20.52 |
CV (%) | 6.2% | 4.9% | 4.7% | 6.9% | 5.4% | 5.7% |
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.001 | 0.138 | 0.303 | 0.530 | 1.091 | 1.601 | 2.100 | 2.345 |
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