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发货周期:现货
博士德生物
cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Human
96T
货号 | FEK0410 |
---|---|
产品名称 | Human IL-6 Quick ELISA Kit |
产品描述 | For quantitative detection of human IL-6 in cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
免疫原 | E.coli, P29-M212 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.3pg/ml |
检测范围 | 4.69pg/ml-300pg/ml |
基因名称 | IL6 |
基因全名 | interleukin 6 |
Uniprot ID | IL6: P05231 |
指标别名 | B cell differentiation factor|B cell stimulatory factor 2|B-cell stimulatory factor 2|BSF 2|BSF-2|BSF2|CDF|CTL differentiation factor|Cytotoxic T cell differentiation factor|Hepatocyte stimulating fact|HGF|HPGF|HSF|Hybridoma growth factor|Hybridoma plasmacytoma growth factor|IFN-beta-2|IFNB2|IL 6|IL-6|IL6|IL6 protein|IL6_HUMAN|Interferon beta 2|Interferon beta-2|Interleukin 6|Interleukin 6 (interferon beta 2)|Interleukin BSF 2|Interleukin-6|Interleukin6 |
背景资料 | The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
FEK0410-CAP | Anti-human IL-6 Pre-coated 96-well Strip Microplate | 1 |
EK0410-ST | human IL-6 standard | 10ng/tube×2 |
FEK0410-H | HRP Linked Anti-human IL-6 Antibody | 6ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | S top solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
CV(%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
Concentration(pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 |
O.D. | 0.002 | 0.059 | 0.106 | 0.226 | 0.428 | 0.736 | 1.372 | 2.279 |
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