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发货周期:现货
博士德生物
cell culture supernates. serum and plasma(heparin, EDTA, citrate).
Mouse
96T
货号 | FEK0495 |
---|---|
产品名称 | Mouse Rantes Quick ELISA Kit |
产品描述 | For quantitative detection of mouse Rantes in cell culture supernates. serum and plasma(heparin, EDTA, citrate). |
免疫原 | E.coli, S24-S91 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | CCL5 |
基因全名 | chemokine (C-C motif) ligand 5 |
Uniprot ID | CCL5: P30882 |
指标别名 | Beta chemokine RANTES|Beta chemokine RANTES precursor|C C motif chemokine 5|CCL 5|CCL5|CCL5_HUMAN|Chemokine (C C motif) ligand 5|Chemokine CC Motif Ligand 5|D17S136E|EoCP|Eosinophil chemotactic cytokine|MGC17164|RANTES(4-68)|Regulated upon activation normally T expressed and presumably secreted|SCYA 5|SCYA5|SIS delta|SIS-delta|SISd|Small inducible cytokine A5|Small inducible cytokine A5 (RANTES)|Small inducible cytokine subfamily A (Cys Cys) member 5|Small-inducible cytokine A5|T cell specific protein p288|T cell specific protein RANTES|T cell specific RANTES protein|T cell-specific protein P228|T-cell-specific protein RANTES|TCP 228|TCP228 |
背景资料 | Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
FEK0495-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
EK0495-ST | mouse Rantes standard | 10ng/tube×2 |
FEK0495-C | mouse Rantes Antibody Cocktail | 6ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | S top solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 29 | 191 | 416 | 26 | 175 | 442 |
Standard deviation | 1.65 | 8.97 | 24.54 | 1.71 | 10.85 | 29.17 |
CV(%) | 5.7% | 4.7% | 5.9% | 6.6% | 6.2% | 6.6% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.016 | 0.164 | 0.255 | 0.450 | 0.831 | 1.445 | 2.087 | 2.262 |
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