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发货周期:现货
博士德生物
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | FEK0326 |
---|---|
产品名称 | Mouse EGF Quick ELISA Kit |
产品描述 | The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Egf with <1 pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, serum, plasma (heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
免疫原 | Expression system for standard: E.coli; Immunogen sequence: N977-R1029 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1 pg/ml |
检测范围 | 7.8 pg/ml - 500 pg/ml |
基因名称 | Egf |
基因全名 | epidermal growth factor |
Uniprot ID | Egf: P01132 |
背景资料 | Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
FEK0326-CAP | Anti- human BDNF Pre-coated 96-well Strip Microplate | 1 |
EK0326-ST | human BDNF standard | 10ng/tube×2 |
FEK0326-H | HRP Linked Anti-human BDNF Antibody | 6ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | S top solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 52 | 277 | 19 | 63 | 274 |
Standard deviation | 1.35 | 3.43 | 15.24 | 0.97 | 5.1 | 18.08 |
CV(%) | 7.5% | 6.6% | 5.5% | 5.1% | 8.1% | 6.6% |
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.045 | 0.190 | 0.322 | 0.554 | 0.989 | 1.746 | 2.328 | 2.403 |
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