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发货周期:现货
博士德生物
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | FEK0975 |
---|---|
产品名称 | Mouse Chitinase 3-Like 1/YKL-40 Quick ELISA Kit |
产品描述 | The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Chi3l1 with <10 pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
免疫原 | Expression system for standard: NS0; Immunogen sequence: Y22-A381 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 62.5 pg/ml - 4,000 pg/ml |
基因名称 | CHI3L1 |
基因全名 | chitinase 3-like 1 (cartilage glycoprotein-39) |
Uniprot ID | CHI3L1: Q61362 |
背景资料 | Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. |
SKU | 名称 | 数量 |
FEK0663-CAP | 96-well plate precoated with anti-Mouse YKL-40 antibody | 1 |
EK0663-ST | lyophilized recombinant Mouse YKL-40 standard | 10ng/tube×2 |
FEK0663-C | Mouse YKL-40 Antibody Cocktail | 6ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 122 | 481 | 2102 | 115 | 474 | 2166 |
Standard deviation | 8.17 | 26.94 | 96.69 | 8.17 | 32.23 | 99.64 |
CV(%) | 6.7% | 5.6% | 4.6% | 7.1% | 6.8% | 4.6% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.039 | 0.084 | 0.128 | 0.225 | 0.414 | 0.769 | 1.345 | 2.160 |
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