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发货周期:现货
博士德生物
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Human
96T
货号 | FEK0555 |
---|---|
产品名称 | Human CCL21/6Ckine/Exodus 2 Quick ELISA Kit |
产品描述 | The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human CCL21 with <12 pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
免疫原 | Expression system for standard: E.coli; Immunogen sequence: S24-P134 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12 pg/ml |
检测范围 | 62.5 pg/ml - 4,000 pg/ml |
基因名称 | CCL21 |
基因全名 | chemokine (C-C motif) ligand 21 |
Uniprot ID | CCL21: O00585 |
背景资料 | Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses. |
SKU | 名称 | 数量 |
FEK0663-CAP | 96-well plate precoated with anti-Human CCL21 antibody | 1 |
EK0663-ST | lyophilized recombinant Human CCL21 standard | 10ng/tube×2 |
FEK0663-C | Human CCL21 Antibody Cocktail | 6ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 117 | 825 | 1412 | 108 | 794 | 1510 |
Standard deviation | 4.56 | 30.53 | 83.31 | 7.02 | 34.94 | 92.11 |
CV (%) | 3.9% | 3.7% | 5.9% | 6.5% | 4.4% | 6.1% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.010 | 0.139 | 0.313 | 0.475 | 0.802 | 1.584 | 2.396 | 2.608 |
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