cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
规格:
96T
产品概况
货号
EK0511-EQ
产品名称
Horse equine TGF Alpha ELISA Kit
产品描述
Sandwich High Sensitivity ELISA kit for Quantitative Detection of horse equine TGF alpha. 96wells/kit, with removable strips.
免疫原
Expression system for standard: E.coli,V40-A89
交叉反应
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
敏感性
<1pg/ml
检测范围
15.6pg/ml-1000pg/ml
基因名称
TGFA
基因全名
Protransforming growth factor alpha
Uniprot ID
P01135
指标别名
Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA;
背景资料
Transforming growth factor alpha(TGF-alpha) is upregulated in some horse equine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to horse equine chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic horse equine TGF-alpha showed similar immunoreactivity when compared with horse equine TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
● 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
● 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.