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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0698 |
---|---|
产品名称 | Human CD27 ELISA Kit |
产品描述 | For quantitative detection of human TNFRSF7 in cell culture supernates and serum. |
免疫原 | NSO, T21-I192 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD27 |
基因全名 | CD27 molecule |
Uniprot ID | P26842 |
指标别名 | CD 27|Cd27|CD27 antigen|CD27 molecule|CD27_HUMAN|CD27L receptor|LPFS2|MGC20393|OTTHUMP00000238557|S152|T cell activation antigen CD27|T cell antivation antigen S152|T-cell activation antigen CD27|T14|TNFRSF 7|TNFRSF7|TNFSF7|Tp 55|Tp55|Tumor necrosis factor receptor superfamily member 7 |
背景资料 | CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0698-CAP | 96-well plate precoated with anti- human TNFRSF7 antibody | 1 |
EK0698-ST | Lyophilized recombinant human TNFRSF7 standard | 10ng/tubex2 |
EK0698-DA | Biotinylated anti- human TNFRSF7 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 103 | 595 | 1421 | 108 | 586 | 1478 |
Standard deviation | 6.79 | 27.37 | 92.36 | 8.2 | 35.74 | 121.19 |
CV(%) | 6.6% | 4.6% | 6.5% | 7.6% | 6.1% | 8.2% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.036 | 0.118 | 0.194 | 0.331 | 0.587 | 1.009 | 1.567 | 2.242 |
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