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发货周期:7-8周
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
Mouse/Rat
96T
货号 | EK2184 |
---|---|
产品名称 | Mouse/Rat DCC ELISA Kit |
产品描述 | For quantitative detection of mouse/rat Dcc in cell culture supernatants, serum and plasma (heparin, EDTA). |
免疫原 | sf21,F32 - N1097 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 23.4 pg/ml - 1,500 pg/ml |
基因名称 | Dcc |
基因全名 | deleted in colorectal carcinoma |
Uniprot ID | P70211/Q63155 |
指标别名 | Dcc; Netrin receptor DCC; Tumor suppressor protein DCC |
背景资料 | Netrin receptor DCC, also known as DCC, or colorectal cancer suppressor is a protein which in humans is encoded by the DCC gene. Predicted to enable identical protein binding activity; netrin receptor activity; and transcription coactivator activity. Involved in postsynaptic modulation of chemical synaptic transmission. Acts upstream of or within generation of neurons. Located in axon. Is integral component of postsynaptic density membrane. Is active in Schaffer collateral - CA1 synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; lung; and retina. Human ortholog(s) of this gene implicated in colorectal cancer; congenital mirror movement disorder; esophageal cancer; and esophagus squamous cell carcinoma. Orthologous to human DCC (DCC netrin 1 receptor). |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2184-CAP | 96-well plate precoated with anti- Mouse/Rat DCC antibody | 1 |
EK2184-ST | Lyophilized recombinant Mouse/Rat DCC standard | 10ng/tubex2 |
EK2184-DA | Biotinylated anti- Mouse/Rat DCC antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 13 | 98 | 265 | 19 | 103 | 282 |
Standard deviation | 0.81 | 6.17 | 15.64 | 0.91 | 5.25 | 15.51 |
CV(%) | 6.2% | 6.3% | 5.9% | 4.8% | 5.1% | 5.5% |
Concentration(pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1,500 |
O.D. | 0.101 | 0.182 | 0.332 | 0.452 | 0.956 | 1.135 | 1.525 | 2.112 |
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