Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
研究类别
1. "Entrez Gene: KRT5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)".2. Eckert RL, Rorke EA (Jun 1988). "The sequence of the human epidermal 58-kD (#5) type II keratin reveals an absence of 5' upstream sequence conservation between coexpressed epidermal keratins". Dna. 7 (5): 337–45.3. Lersch R, Fuchs E (Jan 1988). "Sequence and expression of a type II keratin, K5, in human epidermal cells". Molecular and Cellular Biology. 8 (1): 486–93.
[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00398)|Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Lane 5) or pCMV6-ENTRY KRT5 (Lane 1), KRT6A (Lane 1), KRT6B (Lane 2), KRT6C (Lane 3) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5/6.|Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00398)|Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00398)|Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Right lane)or pCMV6-ENTRY KRT5 (left lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5/6.[/list_product_images]