A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I(51-68aa SYNPATAKEIINVGHSFH), different from the mouse sequence by one amino acid.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participate in a variety of biologic processes like respiration, calcification, acid-base balance etc.
研究类别
1. Lowe N, Edwards YH, Edwards M, Butterworth PH (Nov 1991). "Physical mapping of the human carbonic anhydrase gene cluster on chromosome 8". Genomics 10 (4): 882–8. 2. "Entrez Gene: CA1 carbonic anhydrase I".
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of anti- CA1 antibody (BA4061-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat spleen tissue lysates.
Use rabbit anti- CA1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CA1 at approximately 29KD. The expected band size for CA1 is at 29KD.[/list_product_images]